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Recombinant human ACAA protein

CAT: 0112-ATGP0674-500Size: 500 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0674-500Size:500 µg
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Description
ACAA1 is a member of the thiolase family of enzymes and is involved in lipid metabolism. This protein is localized to the peroxisome and catalyzes the conversion of acyl-CoA and acetyl-CoA to 3-oxoacyl-CoA in the fatty acid oxidation pathway. ACAA1 shows high enzymatic activity in liver, kidney, intestine and white adipose tissue in rats. Deficiency of this enzyme leads to pseudo-Zellweger syndrome. Recombinant human ACAA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
43.8 kDa (419aa) confirmed by MALDI-TOF
Additionnal Information
ACAA, 3-ketoacyl-CoA thiolase peroxisomal, ACAA, PTHIO, THIO, 3-ketoacyl-CoA thiolase, peroxisomal, ATGP0674-10 µg, ATGP0674-20 µg, ATGP0674-50 µg, ATGP0674-100 µg, ATGP0674-250 µg, ATGP0674-500 µg, ATGP0674-1 mg, ATGP0674-10, ATGP0674-20, ATGP0674-50, ATGP0674-100, ATGP0674-250, ATGP0674-500, ATGP0674-1
References & Citations
Bout A., et al. (1991) Biochim Biophys Acta. 1090 (1) :43-51. ; ; Patel S., et al. (2003) Eur Respir J. 22 (5) :755-60.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001598
Uniprot Accession Number
P09110
Species
Human
AA Sequence
MLSGAPQASA ADVVVVHGRR TAICRAGRGG FKDTTPDELL SAVMTAVLKD VNLRPEQLGD ICVGNVLQPG AGAIMARIAQ FLSDIPETVP LSTVNRQCSS GLQAVASIAG GIRNGSYDIG MACGVESMSL ADRGNPGNIT SRLMEKEKAR DCLIPMGITS ENVAERFGIS REKQDTFALA SQQKAARAQS KGCFQAEIVP VTTTVHDDKG TKRSITVTQD EGIRPSTTME GLAKLKPAFK KDGSTTAGNS SQVSDGAAAI LLARRSKAEE LGLPILGVLR SYAVVGVPPD IMGIGPAYAI PVALQKAGLT VSDVDIFEIN EAFASQAAYC VEKLRLPPEK VNPLGGAVAL GHPLGCTGAR QVITLLNELK RRGKRAYGVV SMCIGTGMGA AAVFEYPGN

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