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Recombinant human NUDT5 protein

CAT: 0112-ATGP0633-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0633-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
NuDT5 is a member of the nudix hydrolase family which eliminate toxic nucleotide derivatives from the cell. NuDT5 hydrolyzes ADP-ribose and ADP-mannose in the presence of magnesium, and also hydrolyzes other nucleotide sugars with low activity such as ADP-glucose and diadenosine diphosphate. As a nudix hydrolase, NuDT5 contains a central nudix motif and functions to eliminate toxic nucleotide metabolites from the cell while maintaining the levels of signaling nucleotides. NuDT5 is widely expressed but is most abundant in liver as a homodimer. Recombinant human NuDT5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Nudix hydrolase 5, ADP-sugar pyrophosphatase, 8-oxo-dGDP phosphatase, Nuclear ATP-synthesis protein NUDIX5, Nucleoside diphosphate-linked moiety X motif 5, Nudix motif 5, YSA1H, hYSAH1, NUDIX5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
26.5 kDa (239aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will be appear higher)
Additionnal Information
ADP-sugar Pyrophosphatase, NUDT5, ADP-sugar pyrophosphatase, hYSAH1, YSA1, YSA1H, ADP-sugar pyrophosphatase, HSPC115, Nudix motif 5, NuDT-5, NuDT 5, ATGP0633-10 µg, ATGP0633-20 µg, ATGP0633-50 µg, ATGP0633-100 µg, ATGP0633-250 µg, ATGP0633-500 µg, ATGP0633-1 mg, ATGP0633-10, ATGP0633-20, ATGP0633-50, ATGP0633-100, ATGP0633-250, ATGP0633-500, ATGP0633-1
References & Citations
Yu HN., et al. (2007) Biochem Biophys Res Commun. 354 (3) :764-8.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_054861
Uniprot Accession Number
Q9UKK9
Species
Human
AA Sequence
MESQEPTESS QNGKQYIISE ELISEGKWVK LEKTTYMDPT GKTRTWESVK RTTRKEQTAD GVAVIPVLQR TLHYECIVLV KQFRPPMGGY CIEFPAGLID DGETPEAAAL RELEEETGYK GDIAECSPAV CMDPGLSNCT IHIVTVTING DDAENARPKP KPGDGEFVEV ISLPKNDLLQ RLDALVAEEH LTVDARVYSY ALALKHANAK PFEVPFLKF

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