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Recombinant human GCDH protein

CAT: 0112-ATGP0622-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0622-010Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Glutaryl-CoA dehydrogenase (GCDH) belongs to the acyl-CoA dehydrogenase family. GCDH catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO (2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. This Protein uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. Recombinant GCDH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
45.7 kDa (415aa) confirmed by MALDI-TOF
Additionnal Information
GCDH, Glutaryl-CoA dehydrogenase, ACAD5, GCD, Glutaryl-CoA dehydrogenase, ACAD-5, ACAD 5, ATGP0622-10 µg, ATGP0622-20 µg, ATGP0622-50 µg, ATGP0622-100 µg, ATGP0622-250 µg, ATGP0622-500 µg, ATGP0622-1 mg, ATGP0622-10, ATGP0622-20, ATGP0622-50, ATGP0622-100, ATGP0622-250, ATGP0622-500, ATGP0622-1
References & Citations
Keyser B., et al. (2008) Hum. Mol. Genet. 17:3854-3863; ; Westover JB., et al. (2001) Biochemistry. 40 (46) :14106-14.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 5mM DTT, 200 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_000150
Uniprot Accession Number
Q92947
Species
Human
AA Sequence
MRPEFDWQDP LVLEEQLTTD EILIRDTFRT YCQERLMPRI LLANRNEVFH REIISEMGEL GVLGPTIKGY GCAGVSSVAY GLLARELERV DSGYRSAMSV QSSLVMHPIY AYGSEEQRQK YLPQLAKGEL LGCFGLTEPN SGSDPSSMET RAHYNSSNKS YTLNGTKTWI TNSPMADLFV VWARCEDGCI RGFLLEKGMR GLSAPRIQGK FSLRASATGM IIMDGVEVPE ENVLPGASSL GGPFGCLNNA RYGIAWGVLG ASEFCLHTAR QYALDRMQFG VPLARNQLIQ KKLADMLTEI TLGLHACLQL GRLKDQDKAA PEMVSLLKRN NCGKALDIAR QARDMLGGNG ISDEYHVIRH AMNLEAVNTY EGTHDIHALI LGRAITGIQA FTASK

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