Recombinant human APRT protein

• Description :

  APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.

• Product Name Alternative :

  Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase.

• Expression System :

  E.coli

• Antigen Species :

  Human

• Tag :

  Non-Tagged

• Applications :

  SDS-PAGE

• Concentration :

  1 mg/mL (determined by Bradford assay)

• Purity :

  > 90% by SDS-PAGE

• Molecular Weight :

  19.6 kDa (180aa) confirmed by MALDI-TOF

• Additionnal Information :

  APRT, Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase., ATGP0483-10 µg, ATGP0483-20 µg, ATGP0483-50 µg, ATGP0483-100 µg, ATGP0483-250 µg, ATGP0483-500 µg, ATGP0483-1 mg, ATGP0483-10, ATGP0483-20, ATGP0483-50, ATGP0483-100, ATGP0483-250, ATGP0483-500, ATGP0483-1

• References & Citations :

  Baranowska-Bosiacka I., et al. (2009) Toxicology. 259 (1-2) :77-83.; ; Liang L., et al. (2007) Cancer Res. 67 (5) :1910-7.;

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

• Scientific Category :

  Metabolism

• NCBI Accession Number :

  NP_000476

• Uniprot Accession Number :

  P07741

• Species :

  Human

• AA Sequence :

  MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE