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Recombinant human APRT protein

CAT: 0112-ATGP0483-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0483-020Size:20 µg
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Description
APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase.
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
19.6 kDa (180aa) confirmed by MALDI-TOF
Additionnal Information
APRT, Adenine phosphoribosyltransferase, AMP, Adenine phosphoribosyltransferase AMP diphosphorylase, AMP pyrophosphorylase, DKFZp686D13177, MGC125856, MGC125857, MGC129961, Transphosphoribosidase., ATGP0483-10 µg, ATGP0483-20 µg, ATGP0483-50 µg, ATGP0483-100 µg, ATGP0483-250 µg, ATGP0483-500 µg, ATGP0483-1 mg, ATGP0483-10, ATGP0483-20, ATGP0483-50, ATGP0483-100, ATGP0483-250, ATGP0483-500, ATGP0483-1
References & Citations
Baranowska-Bosiacka I., et al. (2009) Toxicology. 259 (1-2) :77-83.; ; Liang L., et al. (2007) Cancer Res. 67 (5) :1910-7.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Scientific Category
Metabolism
NCBI Accession Number
NP_000476
Uniprot Accession Number
P07741
Species
Human
AA Sequence
MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE

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