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Recombinant human E2-EPF/UBE2S protein

CAT: 0112-ATGP0372-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0372-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins. Recombinant uBE2S protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.9 kDa (258aa) confirmed by MALDI-TOF
Additionnal Information
UBE2S, ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, ubiquitin-conjugating enzyme E2S AA409170, MGC101982, uBE2S, ubiquitin carrier protein S, ubiquitin protein ligase S, ubiquitin conjugating enzyme E2 24 kD, ubiquitin conjugating enzyme E2S, ATGP0372-10 µg, ATGP0372-20 µg, ATGP0372-50 µg, ATGP0372-100 µg, ATGP0372-250 µg, ATGP0372-500 µg, ATGP0372-1 mg, ATGP0372-10, ATGP0372-20, ATGP0372-50, ATGP0372-100, ATGP0372-250, ATGP0372-500, ATGP0372-1
References & Citations
Ye Y., et al. (2009) Nat Rev Mol Cell Biol. 10 (11) :755-64. ; ; Windheim M., et al. (2008) Biochem J. 409 (3) :723-9.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT and 20% glycerol
Scientific Category
Ubiquitination
NCBI Accession Number
NP_055316.2
Uniprot Accession Number
Q16763
Species
Human
AA Sequence
MNSN VENLPPHIIR LVYKEVTTLT ADPPDGIKVF PNEEDLTDLQ VTIEGPEGTP YAGGLFRMKL LLGKDFPASP PKGYFLTKIF HPNVGANGEI CVNVLKRDWT AELGIRHVLL TIKCLLIHPN PESALNEEAG RLLLENYEEY AARARLLTEI HGGAGGPSGR AEAGRALASG TEASSTDPGA PGGPGGAEGP MAKKHAGERD KKLAAKKKTD KKRALRRL

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