Products for Research Use Only
RegisterLogin

Human E2-EPF/UBE2S antibody

CAT: 0112-ATGA0165-100Size: 100 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGA0165-100Size:100 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
UBE2S, also known as ubiquitin-conjugating enzyme E2S, is a member of the ubiquitin-conjugating enzyme family. This protein is able to form a thiol ester linkage with ubiquitin in an ubiquitin activating enzyme-dependent manner, a characteristic property of ubiquitin carrier proteins. It catalyzes the covalent attachment of ubiquitin to other proteins.
Product Name Alternative
Ubiquitin-conjugating enzyme E2S, E2-EPF, E2EPF, EPF5, Ubiquitin-conjugating enzyme S, UBE2S, Ubiquitin carrier protein S, Ubiquitin protein ligase S, Ubiquitin conjugating enzyme E2 24 kD, Ubiquitin conjugating enzyme E2S
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human UBE2S (1-222aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT2C12
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
UBE2S, ATGA0165-10 µg, ATGA0165-20 µg, ATGA0165-50 µg, ATGA0165-100 µg, ATGA0165-250 µg, ATGA0165-500 µg, ATGA0165-1 mg, ATGA0165-10, ATGA0165-20, ATGA0165-50, ATGA0165-100, ATGA0165-250, ATGA0165-500, ATGA0165-1
References & Citations
Ye Y, et al. (2009) Nat Rev Mol Cell Biol, 10 (11) :755-64.; ; Windheim M, et al. (2008) Biochem J, 409 (3) :723-9.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Ubiquitination
NCBI Accession Number
NP_055316.2
Uniprot Accession Number
Q16763
WB Description
The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2S antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: UBE2S Transfected 293T cell lysate;
FACS Description
Flow cytometry analysis of UBE2S in Jurkat cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
Immunofluorescence of human HeLa cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human UBE2S antibody (1:500) with Alexa 488 (Green) .

Related Products

CatalogName
ATGP0372-100Recombinant human E2-EPF/UBE2S protein
ATGP0372-500Recombinant human E2-EPF/UBE2S protein
ATGP0372-020Recombinant human E2-EPF/UBE2S protein
HSP0801-500Recombinant human HSP10/EPF protein
HSP0801-100Recombinant human HSP10/EPF protein
HSP0801-020Recombinant human HSP10/EPF protein
ATGP0894-100Recombinant human E2-25K/UBE2K protein
ATGP0894-010Recombinant human E2-25K/UBE2K protein
ATGP0894-500Recombinant human E2-25K/UBE2K protein
ATGP0894-050Recombinant human E2-25K/UBE2K protein

Alternative Products

CatalogName
34030-05111Human UBE2S Antibody
34030-05121Human UBE2S Antibody (Biotin Conjugate)
34030-05161Human UBE2S AssayLite™ Antibody (APC Conjugate)
34030-05151Human UBE2S AssayLite™ Antibody (RPE Conjugate)
22210-05011Human Apolipoprotein E2 (Apo E2) Antibody
34030-05171Human UBE2S AssayLite™ Antibody (PerCP Conjugate)
34030-05141Human UBE2S AssayLite™ Antibody (FITC Conjugate)
22210-05021Human Apolipoprotein E2 (Apo E2) Antibody (Biotin Conjugate)
22210-05051Human Apolipoprotein E2 (Apo E2) AssayLite™ Antibody (RPE Conjugate)
22210-05071Human Apolipoprotein E2 (Apo E2) AssayLite™ Antibody (PerCP Conjugate)

Popular Products