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Recombinant human ASF1A protein

CAT: 0112-ATGP0296-020Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP0296-020Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146 Description ASF1 anti-silencing function 1 homolog A, also known as ASF1A, belongs to H3/H4 family of histone chaperone proteins. ASF1A protein interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. Deletion of ASF1A in yeast and Drosophila confers sensitivity to various DNA damaging agents and inhibitors of DNA replication, increases genomic instability and sister chromatid exchange, and activates the DNA damage checkpoint. Recombinant human ASF1A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Name Alternative
ASF1 anti-silencing function 1 homolog A, Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146, ASF1 anti-silencing function 1 homolog A Anti silencing function 1A, ASF1 anti silencing function 1 homolog A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
27 kDa (240aa) confirmed by MALDI-TOF
Additionnal Information
ASF1A, ASF1 anti-silencing function 1 homolog A, Histone chaperone ASF1A, CCG1-interacting factor A (CIA), CGI-98, HSPC146, ASF1 anti-silencing function 1 homolog A Anti silencing function 1A, ASF1 anti silencing function 1 homolog A, CCG1 interacting factor A, CGI 98, CIA, hAsf1, hAsf1a, hCIA, Histone chaperone ASF1A, ATGP0296-10 µg, ATGP0296-20 µg, ATGP0296-50 µg, ATGP0296-100 µg, ATGP0296-250 µg, ATGP0296-500 µg, ATGP0296-1 mg, ATGP0296-10, ATGP0296-20, ATGP0296-50, ATGP0296-100, ATGP0296-250, ATGP0296-500, ATGP0296-1
References & Citations
Sanematsu F., et al. (2006) . J Biol Chem. 281 (19) :13817-27; ; Groth A., et al. (2005) . Mol Cell. 17 (2) :301-11;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT , 10% glycerol
Scientific Category
Histone Related
NCBI Accession Number
NP_054753.1
Uniprot Accession Number
Q9Y294
Species
Human
AA Sequence
MAKV QVNNVVVLDN PSPFYNPFQF EITFECIEDL SEDLEWKIIY VGSAESEEYD QVLDSVLVGP VPAGRHMFVF QADAPNPGLI PDADAVGVTV VLITCTYRGQ EFIRVGYYVN NEYTETELRE NPPVKPDFSK LQRNILASNP RVTRFHINWE DNTEKLEDAE SSNPNLQSLL STDALPSASK GWSTSENSLN VMLESHMDCM

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