NIH/3T3 Lysate

CAT: 0112-ATGL0017-100Size: 100 µgDry Ice: NoHazardous: No

CAT#:0112-ATGL0017-100Size:100 µg

Selected

PRICE

AVAILABILITY: InStock

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

1 / 1

Description

NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.

Antigen Species

Mouse

Applications

Concentration

2 mg/mL (determined by BCA assay)

Additionnal Information

NIHT3, ATGL0017-10 µg, ATGL0017-20 µg, ATGL0017-50 µg, ATGL0017-100 µg, ATGL0017-250 µg, ATGL0017-500 µg, ATGL0017-1 mg, ATGL0017-10, ATGL0017-20, ATGL0017-50, ATGL0017-100, ATGL0017-250, ATGL0017-500, ATGL0017-1

Storage Conditions

Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)

Scientific Category

Lysates

Species

Mouse

WB Description

All lanes : NIH/3T3 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;

Preparation

Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

Lysate QC

Western Blot analyzed by both GAPDH and Beta tubulin antibody

Related Products

Catalog	Name
ATGL0016-200	BALB/3T3 Lysate
ATGL0016-500	BALB/3T3 Lysate
ATGL0021-100	3T3-L1 Lysate
ATGL0021-200	3T3-L1 Lysate
ATGL0021-500	3T3-L1 Lysate
ATGL0016-100	BALB/3T3 Lysate
ATGL0002-500	293T Lysate
ATGL0005-100	HepG2 Lysate
ATGL0008-500	A549 Lysate
ATGL0010-100	Ramos Lysate

Alternative Products

Catalog	Name
552129-01	NIH/3T3 Lysate
1282	3T3 (NIH) Lysate
sc-24794	NIH/3T3 (IP) Cell Lysate
sc-24752	Quiescent NIH/3T3 Cell Lysate
sc-2210	NIH/3T3 Whole Cell Lysate
sc-2247	NIH/3T3 + anisomycin Cell Lysate
sc-3804	NIH/3T3 + UV Cell Lysate
sc-24741	NIH/3T3 + forskolin Cell Lysate
sc-3803	NIH/3T3 + PDGF Cell Lysate
MCL-1282	Mouse 3T3 (NIH), embryo lysate

NIH/3T3 Lysate

Related Products

Alternative Products

Popular Products