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THP-1 Lysate

CAT: 0112-ATGL0013-200Size: 200 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGL0013-200Size:200 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
THP-1 cells derived from the peripheral blood of acute monocytic leukaemia. THP-1 cell line can differentiate into macrophage-like cells (phagocytic) and can be used for induction of differentiation studies. When stimulated with PMA cells have been reported to differentialte into a monocytic pathway and release arachidonic acid and prostanoids.
Antigen Species
Human
Applications
WB
Concentration
2 mg/mL (determined by BCA assay)
Additionnal Information
THP1, ATGL0013-10 µg, ATGL0013-20 µg, ATGL0013-50 µg, ATGL0013-100 µg, ATGL0013-250 µg, ATGL0013-500 µg, ATGL0013-1 mg, ATGL0013-10, ATGL0013-20, ATGL0013-50, ATGL0013-100, ATGL0013-250, ATGL0013-500, ATGL0013-1
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Scientific Category
Lysates
Species
Human
WB Description
All lanes : THP-1 Whole Cell Lysate (40ug) ; ; ; ; Lane 1. : Anti-GAPDH antibody (ATGA0181) ; ; Lane 2. : Anti-Beta tubulin antibody (ATGA0196) ;
Preparation
Heat lysate to 95°C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody

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