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GCA cDNA

CAT: 0112-ATGD0225-010Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGD0225-010Size:10 µg
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Description
GCA product, grancalcin, is a calcium-binding protein abundant in neutrophils and macrophages. It belongs to the penta-EF-hand subfamily of proteins which includes sorcin, calpain, and ALG-2. Grancalcin localization is dependent upon calcium and magnesium. In the absence of divalent cation, grancalcin localizes to the cytosolic fraction; with magnesium alone, it partitions with the granule fraction; and in the presence of magnesium and calcium, it associates with both the granule and membrane fractions, suggesting a role for grancalcin in granule-membrane fusion and degranulation.
Product Name Alternative
GCL
Chromosomal Location
2q24.2
Antigen Species
Human
Vector Type
PATGen (puc19-derived cloning vector)
Sequence
ATGGCCTACCCGGGATACGGAGGAGGGTTTGGAAATTTTAGCATTCAGGTGCCAGGAATGCAGATGGGACAGCCAGTGCCAGAAACAGGCCCAGCTATACTCCTCGATGGATACTCTGGGCCAGCATATTCAGACACTTATTCCTCAGCTGGTGACTCCGTGTATACTTACTTCAGTGCTGTTGCTGGACAGGATGGTGAAGTGGATGCTGAAGAACTTCAGAGATGTTTGACACAGTCTGGAATTAATGGAACTTACTCTCCCTTCAGTTTGGAAACCTGCAGAATTATGATTGCCATGTTGGATAGAGATCACACAGGAAAAATGGGATTTAATGCATTCAAAGAGCTATGGGCAGCTCTTAATGCCTGGAAGGAAAACTTCATGACTGTTGATCAAGATGGAAGTGGCACAGTAGAACATCATGAGTTGCGTCAAGCCATTGGTCTTATGGGTTATAGGTTGAGTCCTCAAACATTAACTACTATTGTTAAACGTTATAGCAAGAATGGCAGAATTTTCTTTGATGATTATGTTGCTTGCTGTGTGAAGCTTCGAGCATTGACAGATTTCTTTAGGAAAAGAGACCACTTGCAACAAGGGTCTGCGAATTTCATATATGACGATTTTTTGCAGGGCACTATGGCAATTTGA
Additionnal Information
GCA, GCL, GCA, ATGD0225-10 µg, ATGD0225-20 µg, ATGD0225-50 µg, ATGD0225-100 µg, ATGD0225-250 µg, ATGD0225-500 µg, ATGD0225-1 mg, ATGD0225-10, ATGD0225-20, ATGD0225-50, ATGD0225-100, ATGD0225-250, ATGD0225-500, ATGD0225-1
Storage Conditions
Store the plasmid at -20C.
Formulation
Lyophilized
Scientific Category
Signal Transduction
Omim
607030
NCBI Accession Number
NP_036330.1
Species
Human
Preparation
1. Centrifuge at 7000rpm for 1 minute.; ;2. Carefully open the vial and add 100 µL of sterile water to dissolve the DNA.; ; Each tube contains approximately 10 µg of lyophilized plasmid.
RNA Reference
NM_012198.3
DNA Size
654bp
Vector Description
This shuttle vector contains the complete ORF. It is inseted BamH I to Xho I. The gene insert contains multiple cloning sites which can be used to easily cut and transfer the gene and recombination site into your expression vector.

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