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Human IL-15 antibody

CAT: 0112-ATGA0603-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0603-100Size:100 µL
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Description
Interleukin-15 (IL-15) is a pleiotropic cytokine that plays a crucial role in both innate and adaptive immunity. IL-15 is unique among cytokines due to its involvement in a trans-signaling mechanism. In this process, IL-15 receptor alpha (IL-15Rα) from one subset of cells presents IL-15 to neighboring cells that express IL-2Rβ/γc. This cytokine is produced by a wide variety of cells and tissues. Similar to IL-2, IL-15 stimulates the proliferation of T-lymphocytes and induces the generation of cytotoxic T-lymphocytes (CTLs) and lymphokine-activated killer (LAK) cells.
Product Name Alternative
Interleukin-15, IL15
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human IL-15 (49-162aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT7G9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL
Additionnal Information
ATGA0603-10 µg, ATGA0603-20 µg, ATGA0603-50 µg, ATGA0603-100 µg, ATGA0603-250 µg, ATGA0603-500 µg, ATGA0603-1 mg, ATGA0603-010, ATGA0603-020, ATGA0603-050, ATGA0603-100, ATGA0603-250, ATGA0603-500, ATGA0603-01M
References & Citations
J G Giri., et al. (1994) EMBO. 13 (12) :2822-2830.; ; Lorenzen I., et al. (2006) J Biol Chem. 281 (10) :6642-6647.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology, Hormones & Cytokines
NCBI Accession Number
NP_000576.1
Uniprot Accession Number
P40933
WB Description
The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IL-15 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human IL-15 protein
FACS Description
Flow cytometry analysis of IL-15 in PBMC cells. The cell was stained with ATGA0603 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of IL-15 in HeLa cells. The cell was stained with ATGA0603 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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