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Human EGFR antibody

CAT: 0112-ATGA0548-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0548-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.
Product Name Alternative
Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EGFR (424-605aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT6E3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EGFR, ATGA0548-10 µg, ATGA0548-20 µg, ATGA0548-50 µg, ATGA0548-100 µg, ATGA0548-250 µg, ATGA0548-500 µg, ATGA0548-1 mg, ATGA0548-10, ATGA0548-20, ATGA0548-50, ATGA0548-100, ATGA0548-250, ATGA0548-500, ATGA0548-1
References & Citations
Cohen S, (1964) Nat Cancer Inst Monogr, 13: 13-27.; ; Toyoda H, et al. (1995) J Biol Chem, 270: 7495-500.; ; Carpenter G, et al. (1978) Nature,276: 409-10.
Other References
Tran AV, et al. Targeted and controlled drug delivery by multifunctional mesoporous silica nanoparticles with internal fluorescent conjugates and external polydopamine and graphene oxide layers. (Acta Biomater. 2018) {https://pubmed.ncbi.nlm.nih.gov/29775731/}; ; Tran VA, et al. Multimodal Mesoporous Silica Nanocarriers for Dual Stimuli-Responsive Drug Release and Excellent Photothermal Ablation of Cancer Cells. (Int J Nanomedicine. 2020) {https://pubmed.ncbi.nlm.nih.gov/33116494/}; ; Mohamed Isa ED, et al. Progress in Mesoporous Silica Nanoparticles as Drug Delivery Agents for Cancer Treatment. (Pharmaceutics. 2021) {https://pubmed.ncbi.nlm.nih.gov/33498885/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_005219
Uniprot Accession Number
P00533
WB Description
The cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Hacat cell lysate;
IF Description
ICC/IF analysis of EGFR in HeLa cells. The cell was stained with ATGA0548 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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