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Recombinant human EGF protein

CAT: 0112-EGF0501-050Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0112-EGF0501-050Size:50 µg
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Description
Recombinant human epidermal growth factor (EGF) is a 6. 3 kDa globular protein containing 54 amino acids residues, including 3 intra-molecular disulfide bonds. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. Recombinant EGF was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Specifications
Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cells. The ED50 range is less or equal to 0.1 ng/mL.
Product Name Alternative
Epidermal growth factor, Pro-epidermal growth factor, urogastrone, EGF
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is less or equal to 0.1 ng/mL.
Molecular Weight
6.3 kDa (54aa) confirmed by MALDI-TOF
Additionnal Information
EGF, EGF0501-10 µg, EGF0501-20 µg, EGF0501-50 µg, EGF0501-100 µg, EGF0501-250 µg, EGF0501-500 µg, EGF0501-1 mg, EGF0501-10, EGF0501-20, EGF0501-50, EGF0501-100, EGF0501-250, EGF0501-500, EGF0501-1, epidermal growth factor (beta-urogastrone)
References & Citations
Riese., et al (1998) Bioessays. 20: 41-48.; ; Cohen S., (1983) Cancer. 15: 1787-1791. ; ; Carpenter G., et al (1979) Annu. Rev. Biochem., 48: 193-216.;
Other References
Tazawa H, et al. Chronic inflammation-derived nitric oxide causes conversion of human colonic adenoma cells into adenocarcinoma cells. (Exp Cell Res. 2013) {https://pubmed.ncbi.nlm.nih.gov/23948305/}; ; Park SY, et al. DNAJB1 negatively regulates MIG6 to promote epidermal growth factor receptor signaling. (Biochim Biophys Acta. 2015) {https://pubmed.ncbi.nlm.nih.gov/26239118/}; ; Okuda K, et al. Pivotal role for S-nitrosylation of DNA methyltransferase 3B in epigenetic regulation of tumorigenesis. (Nat Commun. 2023) {https://pubmed.ncbi.nlm.nih.gov/36739439/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001954.2
Uniprot Accession Number
P01133
Species
Human
AA Sequence
MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG ERCQYRDLKW WELR

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