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His-tag antibody

CAT: 0112-ATGA0544-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0544-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag.
Product Name Alternative
6 His epitope tag antibody, Hexa His tag antibody, HHHHHH epitope tag antibody, HHHHHH tag antibody, His tag antibody, Polyhistidine Tag antibody
Host
Mouse
Immunogen
6x His synthetic peptide
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1D6
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
His tag, ATGA0544-10 µg, ATGA0544-20 µg, ATGA0544-50 µg, ATGA0544-100 µg, ATGA0544-250 µg, ATGA0544-500 µg, ATGA0544-1 mg, ATGA0544-10, ATGA0544-20, ATGA0544-50, ATGA0544-100, ATGA0544-250, ATGA0544-500, ATGA0544-1
References & Citations
O Hare, Simura, Gibson; et al. (2006) . Biomolecular Methods. 38 (22) : 873-885.; ; Li Y et al. from the Mariana Trench. Mar Drugs 17: (2019) .; ; Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U. (2013) .;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1: OTUB2-His tag Transfected 293T cell lysate; ; Lane 2: GAD-His tag Transfected 293T cell lysate; ; The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-His tag antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1: Recombinant human TREM1-His tag protein; ; Lane 2: Recombinant human CD34-His tag protein; ; Lane 3: Recombinant human ATG3-His tag protein; ; {2004010491789039.jpg}

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