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Streptavidin antibody

CAT: 0112-AST0904-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-AST0904-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits.
Product Name Alternative
Streptavidin, Streptomyces avidinii streptavidin, SA V1, SA V2, Streptavidin V1, Streptavidin V2.
Host
Mouse
Antigen Species
Human
Immunogen
Recombinant Streptavidin (25-183aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
1C2
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Streptavidin, AST0904-10 µg, AST0904-20 µg, AST0904-50 µg, AST0904-100 µg, AST0904-250 µg, AST0904-500 µg, AST0904-1 mg, AST0904-10, AST0904-20, AST0904-50, AST0904-100, AST0904-250, AST0904-500, AST0904-1
References & Citations
Mark Howarth, et al. (2006) Nature Methods. 3:267-73.; ; Holmberg A., et al. (2005) Electrophoresis. 26 (3) :501-10.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Protein Marker
NCBI Accession Number
CAA27265
Uniprot Accession Number
P22629
WB Description
The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-Streptavidin antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant Streptavidin protein

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