Human MINCLE/CLEC4E antibody

CAT: 0112-ATGA0537-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0537-050Size:50 µL
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Description
MINCLE (macrophage inducible C-type lectin, also called CLEC4E or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. MINCLE expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB) . Recently it was reported that MINCLE is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.
Product Name Alternative
CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CLEC4E (41-219aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT16E3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0537-10 µg, ATGA0537-20 µg, ATGA0537-50 µg, ATGA0537-100 µg, ATGA0537-250 µg, ATGA0537-500 µg, ATGA0537-1 mg, ATGA0537-010, ATGA0537-020, ATGA0537-050, ATGA0537-100, ATGA0537-250, ATGA0537-500, ATGA0537-01M
References & Citations
Ishikawa E, et al. (2009) J Exp Med, 206 (13) :2879-88.; ; Matsunaga I, et al. (2009) J Exp Med, 206 (13) :2865-8.; ; Graham LM, et al. (2009) Cytokine, 48 (1-2) :148-55.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_055173
Uniprot Accession Number
Q9ULY5
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: CLEC4E Transfected 293T cell lysate;
FACS Description
Flow cytometry analysis of CLEC4E in LnCap cells. The cell was stained with ATGA0537 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of CLEC4E in HeLa cells. The cell was stained with ATGA0537 (1:500) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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