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Human Wnt-3a antibody

CAT: 0112-ATGA0520-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0520-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Wingless-type MMTV integration site family, member 3A (Wnt3a) is a member of the Wnt family. The Wnt gene family is representative of a growing class of multifunctioanl factors that play a role in both tumorigenesis and patterning events required for normal differentiation and development. Wnt3a is a ligand for members of the frizzled family of seven transmembrane receptors.
Product Name Alternative
Wnt3a, Wingless-type MMTV integration site family, member 3A
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Wnt3a (19-352aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
3A6
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Wnt3a, ATGA0520-10 µg, ATGA0520-20 µg, ATGA0520-50 µg, ATGA0520-100 µg, ATGA0520-250 µg, ATGA0520-500 µg, ATGA0520-1 mg, ATGA0520-10, ATGA0520-20, ATGA0520-50, ATGA0520-100, ATGA0520-250, ATGA0520-500, ATGA0520-1
References & Citations
Pan W., et al. (2008) Science. 321 (5894) :1350-1353; ; Katoh M., et al. (2002) Int J Oncol. 20 (2) :373-377; ; Smolich B D., et al. (1993) Mol Biol Cell. 4 (12) :1267-1275
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Development
NCBI Accession Number
NP_149122
Uniprot Accession Number
P56704
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Wnt3a antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: Wnt3a transfected 293T cell lysate

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