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Human PDHX antibody

CAT: 0112-ATGA0494-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0494-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP) . This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex.
Product Name Alternative
Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PDHX (54-501aa) purified from E. coli.
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1E11
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PDHX, Pyruvate dehydrogenase protein X component mitochondrial, Pyruvate dehydrogenase protein X component, mitochondrial, DLDBP, E3BP, OPDX, PDX1, proX, ATGA0494-10 µg, ATGA0494-20 µg, ATGA0494-50 µg, ATGA0494-100 µg, ATGA0494-250 µg, ATGA0494-500 µg, ATGA0494-1 mg, ATGA0494-10, ATGA0494-20, ATGA0494-50, ATGA0494-100, ATGA0494-250, ATGA0494-500, ATGA0494-1
References & Citations
Aral B., et al. (1997) Am. J. Hum. Genet. 61: 1318-1326.; ; Hiromasa Y., et al. (2004) J. Biol. Chem. 279: 6921-6933.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_003468
Uniprot Accession Number
O00330
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PDHX antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: A431 cell lysate ; ; Lane 2.: HepG2 cell lysate ; ; Lane 3.: 293T cell lysate ; ; Lane 4.: LnCap cell lysate

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