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Human TOMM20 antibody

CAT: 0112-ATGA0482-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0482-050Size:50 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitochondrial import receptor subunit TOMM20 homolog, also known as TOMM20, belongs to the Tom20 family. The Tom machinery consists of import receptors for the initial binding of cytosolically synthesized preproteins and a general import pore (GIP) for the membrane translocation of various preproteins into the mitochondria. TOMM20 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore.
Product Name Alternative
Translocase of outer mitochondrial membrane 20, Translocase of outer mitochondrial membrane 20 homolog, Translocase of outer mitochondrial membrane 20 homolog type II, Mitochondrial import receptor subunit TOM20 homolog, Mitochondrial 20 kDa outer membrane protein, Outer mitochondrial membrane receptor Tom20, TOM20, MOM19, MAS20
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Tomm20 (25-145aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT1B2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Tomm20, ATGA0482-10 µg, ATGA0482-20 µg, ATGA0482-50 µg, ATGA0482-100 µg, ATGA0482-250 µg, ATGA0482-500 µg, ATGA0482-1 mg, ATGA0482-10, ATGA0482-20, ATGA0482-50, ATGA0482-100, ATGA0482-250, ATGA0482-500, ATGA0482-1
References & Citations
Ahting U., et al. (1999) J Cell Biol. 147: 959-968.; ; Brix J., et al. (1999) J Biol Chem. 274: 16522-16530.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_055580
Uniprot Accession Number
Q15388
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Tomm20 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: A431 cell lysate; ; Lane 4.: K562 cell lysate; ; Lane 5.: A549 cell lysate; ; Lane 6.: 293T cell lysate; ; Lane 7.: MCF7 cell lysate; ; Lane 8.: SK-OV-3 cell lysate; ; Lane 9.: PC3 cell lysate;
FACS Description
Flow cytometry analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray) .
IF Description
ICC/IF analysis of Tomm20 in HeLa cells. The cell was stained with ATGA0482 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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