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Human ACADS antibody

CAT: 0112-ATGA0459-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0459-050Size:50 µL
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Description
ACADS (Acyl-Coenzyme A dehydrogenase), also known as SCAD or ACAD3, is a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency) .
Product Name Alternative
Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human ACADS (25-412aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT8B10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ACADS, Acyl-Coenzyme A dehydrogenase, ACAD3, SCAD, Acyl-Coenzyme A dehydrogenase Acyl Coenzyme A dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, C2 to C3 short chain, Acyl-CoA dehydrogenase, short chain, Acyl-Coenzyme A dehydrogenase, short chain, AI196007, Bcd-1, Bcd1, Butyryl CoA dehydrogenase, EC 1.3.99.2, Short chain acyl CoA dehydrogenase, unsaturated acyl CoA reductase, Short-chain specific acyl-CoA dehydrogenase, mitochondrial., ATGA0459-10 µg, ATGA0459-20 µg, ATGA0459-50 µg, ATGA0459-100 µg, ATGA0459-250 µg, ATGA0459-500 µg, ATGA0459-1 mg, ATGA0459-10, ATGA0459-20, ATGA0459-50, ATGA0459-100, ATGA0459-250, ATGA0459-500, ATGA0459-1
References & Citations
McAndrew RP., et al. (2008) J Biol Chem. 283 (14) : 9435-43. ; ; Tafti M., et al. (2003) Nat Genet. 34 (3) : 320-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
AAH25963
Uniprot Accession Number
P16219
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACADS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HepG2 cell lysate ; ; Lane 2 : LnCap cell lysate
IF Description
ICC/IF analysis of ACADS in Hep3B cells. The cell was stained with ATGA0459 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .ICC/IF analysis of ACADS in A549 cells. The cell was stained with ATGA0459 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .{2004010398103791.jpg}

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