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Recombinant human ACAD8 protein

CAT: 0112-ATGP2421-100Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0112-ATGP2421-100Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Recombinant human ACAD8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Name Alternative
Isobutyryl-CoA dehydrogenase mitochondrial, isobutyryl-CoA dehydrogenase, mitochondrial, ACAD-8, ARC42, Activator-recruited cofactor 42 kDa component, Acyl-CoA dehydrogenase family member 8, IBD
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
45.1 kDa (416aa) confirmed by MALDI-TOF
Additionnal Information
ACAD8, isobutyryl-CoA dehydrogenase mitochondrial, isobutyryl-CoA dehydrogenase, mitochondrial, ACAD-8, ARC42, Activator-recruited cofactor 42 kDa component, Acyl-CoA dehydrogenase family member 8, IBD, ATGP2421-10 µg, ATGP2421-20 µg, ATGP2421-50 µg, ATGP2421-100 µg, ATGP2421-250 µg, ATGP2421-500 µg, ATGP2421-1 mg, ATGP2421-10, ATGP2421-20, ATGP2421-50, ATGP2421-100, ATGP2421-250, ATGP2421-500, ATGP2421-1
References & Citations
Hendrickson, S.L., et al. (2010) PLoS ONE 5 (9), E12862; ; Battaile, K.P., et al. (2004) J. Biol. Chem. 279 (16), 16526-16534;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Scientific Category
Metabolism
NCBI Accession Number
NP_055199
Uniprot Accession Number
Q9UKU7
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSLVQTGHR SLTSCIDPSM GLNEEQKEFQ KVAFDFAARE MAPNMAEWDQ KELFPVDVMR KAAQLGFGGV YIQTDVGGSG LSRLDTSVIF EALATGCTST TAYISIHNMC AWMIDSFGNE EQRHKFCPPL CTMEKFASYC LTEPGSGSDA ASLLTSAKKQ GDHYILNGSK AFISGAGESD IYVVMCRTGG PGPKGISCIV VEKGTPGLSF GKKEKKVGWN SQPTRAVIFE DCAVPVANRI GSEGQGFLIA VRGLNGGRIN IASCSLGAAH ASVILTRDHL NVRKQFGEPL ASNQYLQFTL ADMATRLVAA RLMVRNAAVA LQEERKDAVA LCSMAKLFAT DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG SNEVMRILIS RSLLQE

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