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Human NCS1 antibody

CAT: 0112-ATGA0442-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0442-050Size:50 µL
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Description
NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. This protein regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. It is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Name Alternative
Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human NCS1 (1-190aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT11B8
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
NCS1, Neuronal calcium sensor 1, Frequenin homolog, FLuP, FREQ, Neuronal calcium sensor 1 Flup, Freq, frequenin (Drosophila) homolog, Frequenin-like protein, NCS1, Neuronal Calcium Sensor 1., ATGA0442-10 µg, ATGA0442-20 µg, ATGA0442-50 µg, ATGA0442-100 µg, ATGA0442-250 µg, ATGA0442-500 µg, ATGA0442-1 mg, ATGA0442-10, ATGA0442-20, ATGA0442-50, ATGA0442-100, ATGA0442-250, ATGA0442-500, ATGA0442-1
References & Citations
Nakamura TY., et al (2001) Proc. Natl. Acad. Sci. u.S.A. 98: 12808-12813. ; ; Bahi N., et al (2003) Hum. Mol. Genet. 12: 1415-1425.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_055101
Uniprot Accession Number
P62166
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NCS1 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: K562 cell lysate ; ; Lane 3.: LnCap cell lysate ; ; Lane 4.: HeLa cell lysate
FACS Description
Flow cytometry analysis of NCS1 in A549 cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of NCS1 in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {2004010381410490.jpg}

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