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Human EphA2 antibody

CAT: 0112-ATGA0441-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0441-050Size:50 µL
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Description
EPHA2, also known as ephrin type-A receptor 2, is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. It is implicated as positional labels that may guide the development of neural topographic maps. It has also been found implicated in embryonic patterning, neuronal targeting, vascular development and adult neovascularization.
Product Name Alternative
EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EPHA2 (27-537aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT66G9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EphA2, Ephrin type-A receptor 2, EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, AW545284, EC 2.7.10.1, Eck , Eph receptor A2, EPHA2, EPHA2_HUMAN, Ephrin receptor, Ephrin receptor EphA2, Ephrin type A receptor 2, Ephrin type-A receptor 2, Epithelial cell kinase, Epithelial cell receptor protein tyrosine kinase, Myk 2, Myk2, Sek 2Sek2, Tyrosine protein kinase receptor ECK, Tyrosine-protein kinase receptor ECK, Tyrosine-protein kinase receptor MPK-5, Tyrosine-protein kinase receptor SEK-2, ATGA0441-10 µg, ATGA0441-20 µg, ATGA0441-50 µg, ATGA0441-100 µg, ATGA0441-250 µg, ATGA0441-500 µg, ATGA0441-1 mg, ATGA0441-10, ATGA0441-20, ATGA0441-50, ATGA0441-100, ATGA0441-250, ATGA0441-500, ATGA0441-1
References & Citations
Flanagan JG., et al. (1998) Annu Rev Neurosci. 21: 309-345. ; ; Cheng N., et al. (2002) Cytokine Growth Factor Rev. 13: 75-85.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004422
Uniprot Accession Number
P29317
WB Description
The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : PC3 cell lysate Recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EPHA2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant human EPHA2 extracellular domain ; ; Lane 2 : Recombinant human EPHA2 cytoplasmic domain {ATGA0441-WB2.jpg}
IF Description
ICC/IF analysis of EPHA2 in HeLa cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . ICC/IF analysis of EPHA2 in A431 cell line, stained monoclonal anti-human EPHA2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0441-IF2.jpg}

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