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Human beta 2-Microglobulin/B2M antibody

CAT: 0112-ATGA0439-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0439-050Size:50 µL
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Description
Beta2 microglobulin, also known as B2M, is a component of MHC class I molecules, Involved in the presentation of peptide antigens to the immune system. B2M is a protein found on the surface of many cells and plentiful on the surface of white blood cells. Increased production or destruction of these cells causes B2M levels in the blood to increase. This increase is seen in people with cancers involving white blood cells, but it is particularly meaningful in people newly diagnosed with multiple myeloma. Multiple myeloma is a malignancy (cancer) of a certain kind of white blood cell, called a plasma cell. B2M Testing is done primarily when evaluating a person for certain kinds of cancer affecting white blood cells including chronic lymphocytic leukemia, non-Hodgkin's lymphoma, and multiple myeloma or kidney disease.
Product Name Alternative
Beta-2-microglobulin, CDABP0092, HDCMA22P
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human B2M (21-119aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT101F10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
B2-Microglobulin, Beta-2-microglobulin, CDABP0092, HDCMA22P, ATGA0439-10 µg, ATGA0439-20 µg, ATGA0439-50 µg, ATGA0439-100 µg, ATGA0439-250 µg, ATGA0439-500 µg, ATGA0439-1 mg, ATGA0439-10, ATGA0439-20, ATGA0439-50, ATGA0439-100, ATGA0439-250, ATGA0439-500, ATGA0439-1
References & Citations
Huang WC., et al (2010) J Biol Chem. 285 (11) : 7947-56. ; ; Morabito A., et al. (2009) Hum Immunol. 70 (7) : 492-5.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis. Flow cytometry and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cardiovascular
NCBI Accession Number
NP_004039
Uniprot Accession Number
P61769
WB Description
The lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human B2M antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : HeLa cell lysate ; ; Lane 2 : U937 cell lysate
FACS Description
Flow cytometry analysis of B2M in A431 cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . Flow cytometry analysis of B2M in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) . {ATGA0439-FC2.jpg}
IF Description
ICC/IF analysis of B2M in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human B2M antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0439-IF1b.jpg}

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