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Human Calbindin 2/CALB2 antibody

CAT: 0112-ATGA0435-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0435-050Size:50 µL
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Description
CALB2 is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers.
Product Name Alternative
CR, Calretinin, CALB2, CAL2, CAB29, 29 kDa calbindin
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CALB2 (1-271aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT5C5
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Calretinin, ATGA0435-10 µg, ATGA0435-20 µg, ATGA0435-50 µg, ATGA0435-100 µg, ATGA0435-250 µg, ATGA0435-500 µg, ATGA0435-1 mg, ATGA0435-10, ATGA0435-20, ATGA0435-50, ATGA0435-100, ATGA0435-250, ATGA0435-500, ATGA0435-1
References & Citations
Rogers JH., et al. (1987) J Cell Biol. 105 (3) : 1343-1353.; ; Dreher B., et al. (1996) J. Comp. Neurol. 376 (2) : 223-40.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
AAH15484
Uniprot Accession Number
P22676
WB Description
The tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CALB2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate
IF Description
ICC/IF analysis of CALB2 in HeLa cells. The cell was stained with ATGA0333 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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