Human CapG antibody

CAT: 0112-ATGA0420-050Size: 50 µLDry Ice: NoHazardous: No
CAT#:0112-ATGA0420-050Size:50 µL
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
Macrophage-capping protein, also known as CAPG, belongs to the gelsolin/villin family of actin-regulatory proteins. CAPG is a calcium-sensitive DNA-binding protein that plays a role in macrophage function. It is expressed in macrophages and macrophage-like cells and can localize both to the nucleus and the cytoplasm. This protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments.
Product Name Alternative
Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CAPG (1-348aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1D10
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CapG, Macrophage-capping protein, MCP, AFCP, Macrophage-capping protein Actin capping protein GCAP39, Actin regulatory protein CAP G, CAPG, Capping protein (actin filament) gelsolin like, Capping protein gelsolin like, gCap39, Gelsolin like capping protein, Macrophage capping protein, mbh1, Myc basic motif homolog 1., ATGA0420-10 µg, ATGA0420-20 µg, ATGA0420-50 µg, ATGA0420-100 µg, ATGA0420-250 µg, ATGA0420-500 µg, ATGA0420-1 mg, ATGA0420-10, ATGA0420-20, ATGA0420-50, ATGA0420-100, ATGA0420-250, ATGA0420-500, ATGA0420-1
References & Citations
Kumar A., et al. (2015) Mol Cell Endocrinol. 404: 91-101.; ; Dabiri G A., et al. (1992) J Biol Chem. 267: 16545-16552.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis and ICC/IF to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
AAH00728
Uniprot Accession Number
P40121
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CAPG antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: HeLa cell lysate ; ; Lane 2.: NIH3T3 cell lysate ; ; Lane 3.: Mouse spleen tissue lysate ; ; Lane 4.: THP-1 cell lysate ; ; Lane 5.: Raw 264.7 cell lysate
IF Description
ICC/IF analysis of CAPG in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CAPG antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) . {ATGA0420-IF1b.jpg}

Popular Products