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Human SGTA antibody

CAT: 0112-ATGA0408-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0408-100Size:100 µL
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Description
SGTA, also known as SGT or hSGT, is a ubiquitously expressed protein that contains three TPR protein-protein interaction repeats. This protein is believed to function as a component of the androgen receptor (AR) -chaperone-cochaperone complex, acting as a cochaperone involved in androgen signaling. In addition, SGTA functions as a cochaperone that binds directly to HSC70 and HSP70 and regulates their ATPase activity. On the basis of its role in apoptosis and androgen signaling, SGTA is a potential candidate for PCOS (polycystic ovary syndrome), a disorder characterized by androgen excess, obesity and menstrual disturbances.
Product Name Alternative
Small glutamine-rich tetratricopeptide repeat-containing protein alpha, alphaSGT, hSGT, SGT, Small glutamine-rich tetratricopeptide repeat-containing protein alpha Small glutamine rich tetratricopeptide repeat TPR containing alpha, uBP, Vpu binding protein
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SGTA (1-313aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT19E8
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SGTA, ATGA0408-10 µg, ATGA0408-20 µg, ATGA0408-50 µg, ATGA0408-100 µg, ATGA0408-250 µg, ATGA0408-500 µg, ATGA0408-1 mg, ATGA0408-10, ATGA0408-20, ATGA0408-50, ATGA0408-100, ATGA0408-250, ATGA0408-500, ATGA0408-1
References & Citations
Andreyeva A., et al. (2010) PLoS One. 5: e12018.; ; Cziepluch C., et al. (1998) J Virol. 72 (5) : 4149-56.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Apoptosis
NCBI Accession Number
NP_003012
Uniprot Accession Number
O43765
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SGTA antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: K562 cell lysate ; ; Lane 3.: A549 cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: LNCaP cell lysate; ; Lane 6.: 293T cell lysate
IF Description
ICC/IF analysis of SGTA in Hep3B cells. The cell was stained with ATGA0408 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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