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Human COX5A antibody

CAT: 0112-ATGA0382-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0382-050Size:50 µL
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Description
COX5A, also known as COX, COX-VA and VA, belongs to the cytochrome c oxidase subunit 5A family. Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. COX5A is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Product Name Alternative
Cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human COX5A (42-150aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
AT7A6
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
COX5A, cytochrome c oxidase subunit Va, cytochrome c oxidase subunit Va, COX, COX-VA, VA, ATGA0382-10 µg, ATGA0382-20 µg, ATGA0382-50 µg, ATGA0382-100 µg, ATGA0382-250 µg, ATGA0382-500 µg, ATGA0382-1 mg, ATGA0382-10, ATGA0382-20, ATGA0382-50, ATGA0382-100, ATGA0382-250, ATGA0382-500, ATGA0382-1
References & Citations
Lenka N., et al. (1998) Prog Nucleic Acid Res Mol Biol. 61: 309-344. ; ; Schmidt TR., et al. (2002) Gene. 286: 13-19.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_004246
Uniprot Accession Number
P20674
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human COX5A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1. : A431 cell lysate ; ; Lane 2. : HeLa cell lysate
IF Description
ICC/IF analysis of COX5A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human COX5A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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