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Human VHR/DUSP3 antibody

CAT: 0112-ATGA0374-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0374-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
DUSP3, also known as Dual specificity protein phosphatase 3, is inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli.
Product Name Alternative
Dual specificity protein phosphatase 3, VHR, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase, Vaccinia virus phosphatase VH1-related
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human DUSP3 (1-185aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT9E6
Conjugation
Unconjugated
Applications
ELISA, WB
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
VHR, ATGA0374-10 µg, ATGA0374-20 µg, ATGA0374-50 µg, ATGA0374-100 µg, ATGA0374-250 µg, ATGA0374-500 µg, ATGA0374-1 mg, ATGA0374-10, ATGA0374-20, ATGA0374-50, ATGA0374-100, ATGA0374-250, ATGA0374-500, ATGA0374-1
References & Citations
Sun H. (1998) Mol Biol. 84: 307-318.; ; Folander K., et al. (1995) Genomics. 23 (1) : 295-296.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_004081
Uniprot Accession Number
P51452
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VHR antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: HepG2 cell lysate; ; Lane 3.: Jurkat cell lysate; ; Lane 4.: MCF7 cell lysate; ; Lane 5.: 293T cell lysate; ; Lane 6.: U87MG cell lysate; ; Lane 7.: K562 cell lysate

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