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Human Ras antibody

CAT: 0112-ATGA0350-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0350-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
All RAS protein family members belong to a class of protein called small GTPase, and are involved cellular signal transduction. RAS is 'switched on' by incoming signals, it subsequently switches on other proteins, which ultimately turn on genes involved in cell growth, differentiation and survival. Mutations in RAS genes can lead to the production of permanently activated RAS proteins. This can cause unintended and overactive signaling inside the cell, even in the absence of incoming signals. Because these signals result in cell growth and division, overactive RAS signaling can ultimately lead to cancer. The 3 Ras genes in humans (HRas, KRas, NRas) are the most common oncogenes in human cancer; mutations that permanently activate RAS are found in 20% to 25% of all human tumors and up to 90% in certain types of cancer. RAS inhibitors are being studied as a treatment for cancer, and other diseases with RAS overexpression.
Product Name Alternative
GTPase Kras, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, NS3, RASK2, GTPase Kras, K-RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS 1, NS 3
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human KRAS (1-186aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2F8
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
Ras, ATGA0350-10 µg, ATGA0350-20 µg, ATGA0350-50 µg, ATGA0350-100 µg, ATGA0350-250 µg, ATGA0350-500 µg, ATGA0350-1 mg, ATGA0350-10, ATGA0350-20, ATGA0350-50, ATGA0350-100, ATGA0350-250, ATGA0350-500, ATGA0350-1
References & Citations
McGrath JP., et al. (1983) Nature. 304 (5926) : 501-506.; ; Schubbert S., et al. (2006) Nat Genet. 38 (3) : 331-336.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_203524
Uniprot Accession Number
P01116
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRAS antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : HeLa cell lysate; ; Lane 2. : HepG2 cell lysate; ; Lane 3. : Ramos cell lysate; ; Lane 4. : A549 cell lysate; ; Lane 5. : Balb/3T3 cell lysate The Recombinant proteins (100ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : KRAS recombinant protein (ATGP2062) ; ; Lane 2. : HRAS recombinant protein (ATGP0516) ; ; Lane 3. : NRAS recombinant protein (ATGP0492) {ATGA0350-WB2.jpg}
IF Description
ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0350 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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