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Human pan-RAS antibody

CAT: 0112-ATGA0203-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0203-100Size:100 µL
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Description
The Ras protein is a subfamily of small GTPases that are involved in signal transduction. They are activated by ligand-binding receptor tyrosine kinases such as EGFR, PDGFR, CSF and FGF. These kinases transiently convert RAS-GDP (inactive form) to RAS-GTP, the active form of RAS. The main genes are HRAS, NRAS and KRAS.
Product Name Alternative
Neuroblastoma RAS viral (v-ras) oncogene homolog, GTPase NRas, HRAS1, ALPS4, N-ras, NRAS1, NS6, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, AV095280, N ras, N ras protein part 4, Neuroblastoma RAS viral (v ras) oncogene homolog, OTTHuMP00000013879, Transforming protein N Ras, v ras neuroblastoma RAS viral oncogene homolog
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human NRAS (1-186aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT2G9
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
V ras neuroblastoma RAS viral oncogene homolog, Transforming protein N Ras, OTTHuMP00000013879, NS6, NRAS1, N-ras, Neuroblastoma RAS viral (v-ras) oncogene homolog OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, OTTMuSP00000023521, Neuroblastoma RAS viral (v-ras) oncogene homolog, Neuroblastoma RAS viral (v ras) oncogene homolog, N ras protein part 4, N ras, HRAS1, GTPase NRas, AV095280, ALPS4, RAS, ATGA0203-10 µg, ATGA0203-20 µg, ATGA0203-50 µg, ATGA0203-100 µg, ATGA0203-250 µg, ATGA0203-500 µg, ATGA0203-1 mg, ATGA0203-010, ATGA0203-020, ATGA0203-050, ATGA0203-100, ATGA0203-250, ATGA0203-500, ATGA0203-01M
References & Citations
Rocks O, et al. (2006) . Current Opinion in Cell Biology 18 (4) : 351-7.; ; Wennerberg K, et al. (March 2005) . J. Cell. Sci. 118 (Pt 5) : 843-6.; ; Rotblat B, et al. (2008) . Methods Enzymol 439: 467-89.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002515
Uniprot Accession Number
P01111
WB Description
The cell lysates of 293T (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS (1:500~1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The recombinant proteins (200ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RAS antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Recombinant human NRAS protein; ; Lane 2.: Recombinant human KRAS protein; ; Lane 3.: Recombinant human HRAS protein; ; {ATGA0203-WB2.jpg}
FACS Description
Flow cytometry analysis of RAS in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of RAS in HeLa cells. The cell was stained with ATGA0203 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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