Human EDAR antibody

• Description :

  Tumor necrosis factor receptor superfamily member EDAR is a protein that in humans is encoded by the EDAR gene. EDAR and other genes provide instructions for making proteins that work together during embryonic development. These proteins form part of a signaling pathway that is critical for the interaction between two cell layers, the ectoderm and the mesoderm. In the early embryo, these cell layers form the basis for many of the body's organs and tissues. Ectoderm-mesoderm interactions are essential for the proper formation of several structures that arise from the ectoderm, including the skin, hair, nails, teeth, and sweat glands.

• Product Name Alternative :

  Tumor necrosis factor receptor superfamily member EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1

• Host :

  Mouse

• Antigen Species :

  Human

• Reactivity :

  Human

• Immunogen :

  Recombinant human EDAR (27-448aa) purified from E.coli

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  AT19E8

• Conjugation :

  Unconjugated

• Applications :

  ELISA, WB, FACS

• Purification Method :

  By protein-A affinity chromatography

• Concentration :

  1 mg/mL (determined by BCA assay)

• Additionnal Information :

  EDAR, ATGA0334-10 µg, ATGA0334-20 µg, ATGA0334-50 µg, ATGA0334-100 µg, ATGA0334-250 µg, ATGA0334-500 µg, ATGA0334-1 mg, ATGA0334-10, ATGA0334-20, ATGA0334-50, ATGA0334-100, ATGA0334-250, ATGA0334-500, ATGA0334-1

• References & Citations :

  Monreal AW, et al. (1999) Nat Genet 22 (4) :366-9.; ; Aswegan AL, et al. (1997) Am J Med Genet 72 (4) :462-7.; ; Entrez Gene: EDAR ectodysplasin A receptor.

• Storage Conditions :

  Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

• Formulation :

  Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol

• Applications Notes :

  The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

• Scientific Category :

  Signal Transduction

• NCBI Accession Number :

  NP_071731

• Uniprot Accession Number :

  Q9UNE0

• WB Description :

  The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant protein EDAR ; ; Lane 2 : A549 cell lysate ; ; Lane 3 : Ramos cell lysate

• FACS Description :

  Flow cytometry analysis of EDAR in HeLa cells. The cell was stained with ATGA0334 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)