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Human EDAR antibody

CAT: 0112-ATGA0334-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0334-100Size:100 µL
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Description
Tumor necrosis factor receptor superfamily member EDAR is a protein that in humans is encoded by the EDAR gene. EDAR and other genes provide instructions for making proteins that work together during embryonic development. These proteins form part of a signaling pathway that is critical for the interaction between two cell layers, the ectoderm and the mesoderm. In the early embryo, these cell layers form the basis for many of the body's organs and tissues. Ectoderm-mesoderm interactions are essential for the proper formation of several structures that arise from the ectoderm, including the skin, hair, nails, teeth, and sweat glands.
Product Name Alternative
Tumor necrosis factor receptor superfamily member EDAR, DL, ECTD10A, ECTD10B, ED1R, ED3, ED5, EDA-A1R, EDA1R, EDA3, HRM1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EDAR (27-448aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT19E8
Conjugation
Unconjugated
Applications
ELISA, WB, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EDAR, ATGA0334-10 µg, ATGA0334-20 µg, ATGA0334-50 µg, ATGA0334-100 µg, ATGA0334-250 µg, ATGA0334-500 µg, ATGA0334-1 mg, ATGA0334-10, ATGA0334-20, ATGA0334-50, ATGA0334-100, ATGA0334-250, ATGA0334-500, ATGA0334-1
References & Citations
Monreal AW, et al. (1999) Nat Genet 22 (4) :366-9.; ; Aswegan AL, et al. (1997) Am J Med Genet 72 (4) :462-7.; ; Entrez Gene: EDAR ectodysplasin A receptor.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_071731
Uniprot Accession Number
Q9UNE0
WB Description
The recombinant protein (50ng) and cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EDAR antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1 : Recombinant protein EDAR ; ; Lane 2 : A549 cell lysate ; ; Lane 3 : Ramos cell lysate
FACS Description
Flow cytometry analysis of EDAR in HeLa cells. The cell was stained with ATGA0334 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray)

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