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Human CKMT1A antibody

CAT: 0112-ATGA0296-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0296-100Size:100 µL
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Description
CKMT1A, also known as Creatine kinase, mitochondrial 1A, is 417 amino acid protein. Mitochondrial creatine kinase (MtCK) exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK (CKMT1) . It is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis.
Product Name Alternative
Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human CKMT1A (40-417aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT17A2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CKMT1A, Creatine kinase u-type mitochondrial, Creatine kinase u-type, mitochondrial, CKMT1; CKMT1B; uMTCK, ATGA0296-10 µg, ATGA0296-20 µg, ATGA0296-50 µg, ATGA0296-100 µg, ATGA0296-250 µg, ATGA0296-500 µg, ATGA0296-1 mg, ATGA0296-10, ATGA0296-20, ATGA0296-50, ATGA0296-100, ATGA0296-250, ATGA0296-500, ATGA0296-1
References & Citations
Haas. R.C., et al. (1989) J Biol Chem 264 (5) : 2890-2897 ; ; Stachowiak. O., et al. (1998) Mol Cell Biochem 184 (1-2) : 141-151; ; Lipskaya. T.Y. (2001) Biochemistry Mosc 66 (10) : 1098-1111
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1000.
Scientific Category
Apoptosis
NCBI Accession Number
NP_001015001
Uniprot Accession Number
P12532
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CKMT1A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: HeLa cell lysate
FACS Description
Flow cytometry analysis of CKMT1A in HeLa cell line, staining at 2-5ug for 1x106cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of CKMT1A in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CKMT1A antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) .

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