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Human PARP2 antibody

CAT: 0112-ATGA0270-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0270-050Size:50 µL
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Description
PARP2 is poly (ADP-ribosyl) transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly (ADP-ribosyl) ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found.
Product Name Alternative
Poly (ADP-ribose) polymerase 2, ADPRT2, ADPRTL2, ADPRTL3, ARTD2, pADPRT-2, PARP-2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human PARP2 (233-583aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
AT29G4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, FACS
Purification Method
By protein-A affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
PARP2, ATGA0270-10 µg, ATGA0270-20 µg, ATGA0270-50 µg, ATGA0270-100 µg, ATGA0270-250 µg, ATGA0270-500 µg, ATGA0270-1 mg, ATGA0270-10, ATGA0270-20, ATGA0270-50, ATGA0270-100, ATGA0270-250, ATGA0270-500, ATGA0270-1
References & Citations
Ame JC, Rolli V, Schreiber V et al. (1999) . J. Biol. Chem. 274 (25) : 17860-8.; ; Schreiber V, Ame JC, Dolle P et al. (2002) . J. Biol. Chem. 277 (25) : 23028-36.; ; Maeda Y, Hunter TC, Loudy DE et al. (2006) . J. Biol. Chem. 281 (14) : 9600-6.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and FACS analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_005475
Uniprot Accession Number
Q9UGN5
WB Description
The cell lysate of HeLa (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500 ~ 1:5000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PARP2 antibody (1:500) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : Raji cell lysate; ; Lane 2. : NIH-3T3 cell lysate; ; {ATGA0270-WB2.jpg}
FACS Description
Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with ATGA0270 at 2-5ug for 1x10^6cells (red) . A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black) .
IF Description
ICC/IF analysis of PARP2 in HeLa cells. The cell was stained with ATGA0270 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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