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Human EGFR antibody

CAT: 0112-ATGA0212-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0212-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Epidermal growth factor receptor (EGFR) is a 170 kDa membrane protein first identified as a binding partner of EGF. Also, EGFR phosphorylated itself (autophosphorylation) in response to EGF treatment. The overexpression of EGFR in various epithelial tumors was widely reported and it has been substantiated that deregulation of EGFR itself and its signaling pathways an important role in human cancers.
Product Name Alternative
Epidermal growth factor receptor, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, ERRP, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, erythroblastic leukemia viral (v-erb-b) oncogene homolog (avian)
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EGFR (424-605aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT2H8
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EGFR, ATGA0212-10 µg, ATGA0212-20 µg, ATGA0212-50 µg, ATGA0212-100 µg, ATGA0212-250 µg, ATGA0212-500 µg, ATGA0212-1 mg, ATGA0212-10, ATGA0212-20, ATGA0212-50, ATGA0212-100, ATGA0212-250, ATGA0212-500, ATGA0212-1
References & Citations
Cohen S, (1964) Nat Cancer Inst Monogr, 13: 13-27. ; ; Toyoda H, et al. (1995) J Biol Chem, 270: 7495-500. ; ; Carpenter G, et al. (1978) Nature,276: 409-10.
Other References
Hoshi H, et al. Construction of a novel cell-based assay for the evaluation of anti-EGFR drug efficacy against EGFR mutation. (Oncol Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/27840973/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Cancer
NCBI Accession Number
NP_005219
Uniprot Accession Number
P00533
WB Description
The cell lysates of HaCaT were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EGFR (1:250) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IF Description
Immunofluorescence of HaCaT cells stained with Hoechst 3342 (Blue) or phalloidin-TRITC for nucleus or actin staining, respectably. monoclonal anti-human EGFR antibody (1:250) was stained with alexa 488 (green) .

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