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Human MINCLE/CLEC4E antibody

CAT: 0112-ATGA0175-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0175-100Size:100 µL
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Description
CLEC4E (macrophage inducible C-type lectin, also called MINCLE or CLECSF9), which is a diverse family of protein which was originally defined by their ability to recognize a wide range of ligand of carbohydrate structure. CLEC4E expressed in macrophages subjected to several types of stress. It plays an essential role in response to trehalose-6, 6'-dimycolate (TDM) and activated by a synthetic analogue, trehalose dibehenate (TDB) . Recently it was reported that CLEC4E is associated with an immunoreceptor tyrosine-based activation motif-containing Fc receptor gamma chain (FcRgamma) and functions as an activating receptor for damaged self- and non-self-pathogenic fungi.
Product Name Alternative
CLEC4E, CLECSF9, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MINCLE (41-219aa) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT16E3
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
CLEC 4E, CLEC4E, CLECSF9, C-type lectin domain family 4, member E, C-type lectin domain family 4 member E, C-type lectin superfamily member 9, Macrophage lectin 2, Macrophage-inducible C-type lectin, MINCLE, NtmntanCLEC4E Ege lectin 2, ATGA0175-10 µg, ATGA0175-20 µg, ATGA0175-50 µg, ATGA0175-100 µg, ATGA0175-250 µg, ATGA0175-500 µg, ATGA0175-1 mg, ATGA0175-010, ATGA0175-020, ATGA0175-050, ATGA0175-100, ATGA0175-250, ATGA0175-500, ATGA0175-01M
References & Citations
Ishikawa E, et al. (2009) J Exp Med, 206 (13) :2879-88.; ; Matsunaga I, et al. (2009) J Exp Med, 206 (13) :2865-8.; ; Graham LM, et al. (2009) Cytokine, 48 (1-2) :148-55.
Other References
Seifert L, et al. The necrosome promotes pancreatic oncogenesis via CXCL1 and Mincle-induced immune suppression. (Nature. 2016) {https://pubmed.ncbi.nlm.nih.gov/27049944/}; ; Zhao X, et al. Activation of C-Type Lectin Receptor and (RIG) -I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages. (J Infect Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/31562757/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Immunology
NCBI Accession Number
NP_055173
Uniprot Accession Number
Q9ULY5
WB Description
The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CLEC4E antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1. : 293T cell lysate; ; Lane 2. : MINCLE transfected 293T cell lysate
IHC description
Paraffin embedded sections of colorectal cancer tissue were incubated with anti-human CLEC4E antibody (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) .
FACS Description
Flow cytometry analysis of CLEC4E in LNCap cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
Immunofluorescence of human HeLa cells stained with monoclonal anti-human CLEC4E antibody (1:500) with Texas Red (Red) . Nucleus was stained by Hoechst 33342 (Blue) .

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