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Human MAT2A antibody

CAT: 0112-ATGA0138-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ATGA0138-100Size:100 µL
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Description
Methionine adenosyltransferase II (MAT II) is a key enzyme in cellular metabolism and catalyzes the formation of S-adenosylmethionine (SAMe) from L-methionine and ATP. MAT2A is expressed in extrahepatic tissues. In liver, MAT2A expression associates with growth, dedifferentiation, and cancer.
Product Name Alternative
Methionine adenosyltransferase II alpha, MATA2, MATII, SAMS2, Methionine adenosyltransferase II alpha, MAT2A, Methionine adenosyltransferase II, alpha AdoMet synthetase, AdoMet synthetase 2, AMS 2, MAT II, MATA 2, Methionine adenosyltransferase, Methionine adenosyltransferase 2, SAMS 2, Methionine adenosyltransferase II, S adenosylmethionine synthetase gamma form, S adenosylmethionine synthetase isoform type 2, S-adenosylmethionine synthetase isoform type 2
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MAT2A (1-395aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT3A2
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
MAT2A, ATGA0138-10 µg, ATGA0138-20 µg, ATGA0138-50 µg, ATGA0138-100 µg, ATGA0138-250 µg, ATGA0138-500 µg, ATGA0138-1 mg, ATGA0138-10, ATGA0138-20, ATGA0138-50, ATGA0138-100, ATGA0138-250, ATGA0138-500, ATGA0138-1
References & Citations
Ramani K, et al., (2008) Hepatology 47 (2) :521-531.; ; Chen H, et al., (2007) Gastroenterology 133 (1) :207-218.
Other References
Ikeda S, et al, Kume S. Role of methionine adenosyltransferase 2A in bovine preimplantation development and its associated genomic regions. (Sci Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/28630431/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Metabolism
NCBI Accession Number
NP_005902
Uniprot Accession Number
P31153
WB Description
The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAT2A antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: HeLa cell lysate; ; Lane 2.: Ramos cell lysate; ; Lane 3.: 293T cell lysate; ; Lane 4.: Jurkat cell lysate; ; Lane 5.: NIH-3T3 cell lysate;
IHC description
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human MAT2A (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
IF Description
ICC/IF analysis of MAT2A in HeLa cells. The cell was stained with ATGA0138 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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