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Human SNAP25 antibody

CAT: 0112-ASN0720-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ASN0720-100Size:100 µL
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Description
The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.
Product Name Alternative
Synaptosomal-associated protein 25 isoform SNAP25B, SNAP25, SNAP, SNAP25, Synaptosomal-associated protein, His- tag, SNAP-25, Synaptosomal-associated 25 kDa protein, Super protein, SuP, Synaptosomal-associated protein 25, Synaptosomal-associated protein 25 isoform SNAP25B bA416N4.2, Bdr, dJ1068F16.2, FLJ23079, HGNC:11132, MGC105414, MGC139754, Resistance to inhibitors of cholinesterase 4 homolog, RIC 4, RIC4, SEC9, SNAP 25, SNAP-25B, SNP 25, sp, Synaptosomal associated 25 kDa protein, Synaptosomal associated protein, Synaptosomal Associated Protein 25, Synaptosomal associated protein 25kDa
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human SNAP25 (1-206aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
400000000000
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
SNAP25, ASN0720-10 µg, ASN0720-20 µg, ASN0720-50 µg, ASN0720-100 µg, ASN0720-250 µg, ASN0720-500 µg, ASN0720-1 mg, ASN0720-10, ASN0720-20, ASN0720-50, ASN0720-100, ASN0720-250, ASN0720-500, ASN0720-1
References & Citations
Schulz JR et al., (1998) J Biol Chem 273 (38) :24355-9.; ; Tafoya LC et al., (2006) J Neurosci 26 (30) :7826-38.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Neuroscience
NCBI Accession Number
NP_570824
Uniprot Accession Number
P60880
WB Description
The tissue lysate (30ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:2000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: Mouse brain tissue lysate The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: SNAP25 Transfected 293T cell lysate {2004010049531236.jpg}
IF Description
ICC/IF analysis of SNAP25 in U87MG cells. The cell was stained with ASN0720 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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