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Human NPM1 antibody

CAT: 0112-ANP0626-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-ANP0626-100Size:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleophosmin (NPM; also known as B23, NO38, or numatrin) is a nucleolar phosphoprotein that binds the tumor suppressors p53 and p19Arf and is thought to be indispensable for ribogenesis, cell proliferation, and survival after DNA damage. Nucleophosmin is upregulated in many cancer cells.
Product Name Alternative
Nucleophosmin (nucleolar phosphoprotein B23, numatrin), B23, NPM, MGC104254, nucleolar phosphoprotein B23, numatrin, nucleophosmin/nucleoplasmin family, member 1
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human NPM (81-294aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
5000
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC, FACS
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
NPM1, ANP0626-10 µg, ANP0626-20 µg, ANP0626-50 µg, ANP0626-100 µg, ANP0626-250 µg, ANP0626-500 µg, ANP0626-1 mg, ANP0626-10, ANP0626-20, ANP0626-50, ANP0626-100, ANP0626-250, ANP0626-500, ANP0626-1
References & Citations
Maiguel DA et al., (2004) Mol cell Biol 24:3703-3711.; ; Colombo Emanuela et al., (2005) Mol cell Biol 25:8874-8886.
Other References
Huang M, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. (Oncotarget. 2016) {https://pubmed.ncbi.nlm.nih.gov/27732946/}
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF, FACS and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002511
Uniprot Accession Number
P06748
WB Description
The HeLa and HepG2 cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NPM (1:1,000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IHC description
This image shows paraffin-embedded human palatine tonsil tissue sample stained with anti-NPM antibody (5E3) at 1:100 dilution.
FACS Description
Flow cytometry analysis of NPM in HeLa cell line, staining at 2-5ug for 1x10^6cells (red line) . The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line) .
IF Description
ICC/IF analysis of NPM in HeLa cells. The cell was stained with ANP0626 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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