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Human HSP60 antibody

CAT: 0112-AHS0815-050Size: 50 µLDry Ice: NoHazardous: No
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CAT#:0112-AHS0815-050Size:50 µL
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Description
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. HSP60 is the ~60kDa mammalian equivalent to GroEL of E. coli. Process of HSP60 is regulated by the cochaperonin HSP10, a single heptameric ring of ~10kD subunits that forms a complex with HSP60. HSP10 coordinates the ATPase activity of the HSP60 subunits to allow the release of bound polypeptide in a manner productive for folding.
Product Name Alternative
Heat shock 60kDa protein 1, CPN60, GroEL, HSP65, SPG13, HuCHA60, Heat shock 60kDa protein 1, HSP60, Heat shock 60kDa protein 1 60 kDa chaperonin, GroEL, E, coli, homolog of, 60 kDa heat shock protein mitochondrial, 60kDa, cb863, Chaperonin, Chaperonin 60, Chaperonin, 60-KD, CPN 60, fa04a05, fb22d10, fi27b05, GroEL Homolog, Heat shock 60kD protein 1 (chaperonin), Heat shock 60kD protein 1 chaperonin, heat shock 60kDa protein 1 (chaperonin), Heat shock protein 1 (chaperonin), Heat Shock Protein 60, Heat shock protein 65, HLD4, Hsp 60, HSP 65, HSPD 1, HSPD1, HuCHA60, id:ibd2197, Spastic paraplegia 13 Mitochondrial matrix protein P1, P60 lymphocyte protein, sb:cb144, Short heat shock protein 60 Hsp60s1, Spastic paraplegia 13 (autosomal dominant), SPG 13, wu:fa04a05, wu:fb22d10, wu:fi04a12, wu:fi27b05.
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human Hsp60 (1-573aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG1 κ
Clone
20000
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
HSP60, AHS0815-10 µg, AHS0815-20 µg, AHS0815-50 µg, AHS0815-100 µg, AHS0815-250 µg, AHS0815-500 µg, AHS0815-1 mg, AHS0815-10, AHS0815-20, AHS0815-50, AHS0815-100, AHS0815-250, AHS0815-500, AHS0815-1
References & Citations
Cheng MY, et al., (1990) Nature. 348:455-8; ; Ghosh JC, et al., (2008) J Biol Chem. 283 (8) :5188-94
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot, ICC/IF and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_002147
Uniprot Accession Number
P10809
WB Description
Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp60 (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.; ; Lane 1.: 293T cell lysate; ; Lane 2.: HeLa cell lysate; ; Lane 3.: A549 cell lysate
IHC description
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human Hsp60 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
IF Description
ICC/IF analysis of Hsp60 in HeLa cells. The cell was stained with AHS0815 (1:100) . The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue) .

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