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Human EphA2 antibody

CAT: 0112-AEA0909-100Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0112-AEA0909-100Size:100 µL
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Description
The Eph family of receptors is the largest known subfamily of receptor tyrosine kinases (RTk) . Eph RTks and their ligands, the ephrins, are frequently overexpressed in a variety of cancers and tumor cell lines. One Eph family member, EphA2, is also overexpressed in a wide variety of cancers, including breast, small-cell lung and gastrointestinal cancers, melanomas, and neuroblastomas. The ephrin-Eph interactions are important in development, especially in cell-cell interactions involved in nervous system patterning and possibly in cancer.
Product Name Alternative
EPH receptor A2, Ephrin type-A receptor 2, EPHA2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ARCC2, CTPA, CTPP1, CTRCT6
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human EphA2 (559-976aa) purified from E. coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
3D7
Conjugation
Unconjugated
Applications
ELISA, WB, IHC
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
EphA2, Ephrin receptor EphA2, epoxide hydrolase 1 microsomal (xenobiotic), AEA0909-10 µg, AEA0909-20 µg, AEA0909-50 µg, AEA0909-100 µg, AEA0909-250 µg, AEA0909-500 µg, AEA0909-1 mg, AEA0909-10, AEA0909-20, AEA0909-50, AEA0909-100, AEA0909-250, AEA0909-500, AEA0909-1
References & Citations
Ireton RC, Chen J: (2005) Curr Cancer Drug Tarqets. 5 (3), 149-57; ; Nakamoto M, Berqemann AD: (2002) Microsc Res Tech. 59 (1), 58-67
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and IHC analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_004422
Uniprot Accession Number
P29317
WB Description
The cell lysates (10 µg) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human EphA2 antibody (1:1000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. ; ; Lane 1.: 293T cell lysate ; ; Lane 2.: EphA2 extracellular domain transfected 293T cell lysate ; ; Lane 3.: EphA2 cytoplasmic domain transfected 293T cell lysate
IHC description
Paraffin embedded sections of human colon cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Paraffin embedded sections of human gastric cancer tissue were incubated with anti-human EphA2 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) {AEA0909-IHC1b.jpg}

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