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CRM003 E.coli & Coliform Chromogenic Medium (ECC)

CAT: 0938-CRM003Size: 1 LDry Ice: NoHazardous: No
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CAT#:0938-CRM003Size:1 L
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Assay Principle
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate were mixed occurrence of coliforms and E. coli enzyme corresponding specific reactions, hydrolysis of the substrate, the release of the color groups, in a pale yellow plates coliforms appears orange-red colonies while E.coli appears blue-green colonies. Formulation (per liter) : Peptone 15.0g Yeast extract powder 3.0g Sodium chloride: 5.0g Sodium lauryl sulfate: 0.1g Agar: 12.0g Mixed chromogenic substrate: 6.77g Final pH 7.0 ± 0.2
Assay Protocol
1.Suspend 41.9g of the product, adding , 1.0 L of distilled or deionized water, heated to boiling stir until completely dissolved, dispensing into flask, 115 °C autoclaved 10minutes. 2.Take 25.0g or 25.0mL of sample with sterile procedures, added to the flask containing 225.0mL of sterile phosphate buffered saline (or saline), shaken thoroughly homogenized with a homogenizer or a 1:10 dilution of 1min solution, diluted 1:10 and then continue to select the appropriate serial dilutions of three, the two plates inoculated with each dilution, poured dissolved by heating and cooled to about 45 °C medium. 3.Observe the results.
Storage Conditions
Keep container tightly closed, store in a cool, dry place, away from bright light.
Quality Control
This product appears light yellow after pouring on plate, these strains were inoculated after 36 ± 1 °C 18 ~ 24h culture growth in the following table. Bacteria name bacteria NO. growth situation feature Escherichia coli ATCC25922 good blue-green colonies Citrobacter ATCC8090 good orange-red colonies Salmonella typhimurium CMCC50115 good colorless colonies Enterococcus faecalis ATCC29212 suppressed ----
Recommended Usage
For the rapid detection and of coliforms and E. coli.

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