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CRM001 Coliform Chromogenic Medium

CAT: 0938-CRM001Size: 1 LDry Ice: NoHazardous: No
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CAT#:0938-CRM001Size:1 L
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Assay Principle
Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups produce green colonies on the light yellow plate. Formulation (per liter) : Peptone :10g Yeast extract powder: 3g sodium chloride:5g sodium lauryl sulfate:0.1g Agar :12g Chromogenic substrate 2.7g Final pH 7.0 ± 0.2
Assay Protocol
1. Suspend 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving. 2.Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) which is shaken thoroughly homogenized with a homogenizer for 1min into a 1:10 dilution, then continue to make for 1:10 dilution. select 3 of appropriate serial dilutions, each dilution was inoculated for 2 plates. 3. Pouring method: Cooled in a water bath to about 50 °C, sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, cultivating at 37 °C for 24 hours. 4.Using surface inoculation: cooled to about 50 °C, shake devoted to the medium in sterile petri dish. stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 °C) . The sample was streaked method or filter method, cultivating 37 °C for 24 hours. 5.observe the results.
Storage Conditions
Keep container tightly closed, store in a cool, dry place, away from bright light.
Quality Control
This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 °C 18 ~ 24h culture growth in the following table. Strains codes growth of bacteria feature Escherichia coli ATCC25922 good green colonies Citrobacter ATCC8090 good green colonies Salmonella typhimurium CMCC50115 good colorless colonies Enterococcus faecalis ATCC29212 suppressed -----
Recommended Usage
For the rapid detection and enumeration of coliform bacteria.

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