EB3 Antibody

• Background:

  The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs) . These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP) . The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

• Synonyms:

  Microtubule RP/EB, MAPRE3, APC, EB3, End-binding. RP3

• Swiss Prot:

  Q9UPY8

• Host:

  Mouse

• Cross Reactivity:

  Human, Mouse, Rat

• Target:

  EB3

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  KT53

• Conjugation:

  Unconjugated

• Source:

  Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.

• Applications:

  WB

• Purification:

  Purified by Protein G.

• Dilution:

  WB (1:300-5000)

• Buffer:

  PBS + 0.05% NaN3

• Modification:

  Unmodified

• Storage Conditions:

  Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

• Specificity:

  The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.