DPH2 Polyclonal Antibody

• Background:

  DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle; moderate expression in heart, small intestine, liver, pancreas, testis and colon; and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.Tissue specificity:Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.

• Synonyms:

  Diphthamide biosynthesis like protein 2; Diphthamide biosynthesis protein 2; Diphthamide biosynthesis protein 2 homolog like 2; Diphthamide biosynthesis protein 2 homolog-like 2; Diptheria toxin resistance protein required for diphthamide biosynthesis like 2; Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae ; DPH 2; DPH2; DPH2 homolog; DPH2 homolog S. cerevisiae; DPH2 like 2; DPH2 like 2 S. cerevisiae; DPH2-like 2; DPH2_HUMAN; DPH2L2; HsDph2; OTTHUMP00000010007; Protein DPH2 homolog.

• Gene ID:

  1802

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Immunogen:

  401-489/489

• Target:

  DPH2

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic peptide derived from human DPH2

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  1802

• Predicted Cross Reactivity:

  Mouse, Rat, Dog, Cow, Sheep, Horse, Chicken, Rabbit