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Phospho-GIT1 (Tyr510) Polyclonal Antibody

CAT: 0498-bs-5374RSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-5374RSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. Involved in the regulation of cytokinesis; the function may involve SDCCAG3 and PTPN13 (By similarity) .
Synonyms
ARF GTPase-activating protein GIT1; ARF GAP GIT1; Cool-associated and tyrosine-phosphorylated protein 1; CAT-1; CAT1; G protein-coupled receptor kinase-interactor 1; GRK-interacting protein 1; GIT1
Gene ID
28964
Swiss Prot
Q9Y2X7
Modification Site
Tyr510
Cellular Locus
Cytoplasm, Cell membrane
Host
Rabbit
Cross Reactivity
Mouse
Target
Phospho-GIT1 (Tyr510)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr510
Applications
WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
28964
Predicted Cross Reactivity
Human, Rat, Cow, Pig, Chicken

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