Phospho-GIT1 (Tyr545) Polyclonal Antibody

• Background:

  Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist (1,2) . G protein-coupled receptor kinases (GRKs) are activated by activated G protein-coupled receptors, and they function to phosphorylate and inactivate cell surface receptors in the heterotrimeric G protein signaling cascade (3,4) . GIT1 (for GRK-interactor 1) and GIT2 are GTPase-activating proteins (GAP) for members of the ADP ribosylation factor (ARF) family of small GTP-binding proteins, which are involved in vesicular trafficking (5,6) . GIT1 overexpression results in reduced internalization and resensitization of b2-adrenergic receptor, thus reducing b2-adrenergic receptor signaling (5) .

• Synonyms:

  GIT1 phospho Tyr545; p-GIT1 Tyr545; ARF GAP GIT1; ARF GTPase activating protein GIT1; CAT 1; Cat1; Cool associated and tyrosine phosphorylated protein 1; G protein coupled receptor kinase interactor 1; GRK interacting protein 1.

• Gene ID:

  28964

• Modification Site:

  Tyr545

• Cellular Locus:

  Cytoplasm, Cell membrane

• Host:

  Rabbit

• Target:

  Phospho-GIT1 (Tyr545)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Source:

  KLH conjugated synthetic phosphopeptide derived from human GIT1 around the phosphorylation site of Tyr545

• Applications:

  WB, ELISA, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Phosphorylation

• Storage Conditions:

  Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

• Gene ID URL:

  28964

• Predicted Cross Reactivity:

  Human, Mouse, Rat, Cow, Pig