TIP5 Polyclonal Antibody

• Background:

  Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing.

• Synonyms:

  Bromodomain adjacent to zinc finger domain protein 2A, BAZ2A, Transcription termination factor I-interacting protein 5, TTF-I-interacting protein 5, Tip5, hWALp3

• Gene ID:

  11176

• Swiss Prot:

  Q9UIF9

• Host:

  Rabbit

• Cross Reactivity:

  Human, Mouse

• Target:

  TIP-5

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Source:

  Polyclonal antibody raised in rabbit against TIP5 (Transcription termination factor I-interacting protein 5), using the recombinant protein

• Applications:

  WB, ELISA

• Purification:

  Whole antiserum from rabbit containing 0.05% azide

• Concentration:

  Not Determined

• Dilution:

  WB (1:300-5000), ELISA (1:500-1000)

• Buffer:

  0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C for 12 months.

• Gene ID URL:

  11176