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Phospho-PPAR alpha (Ser12) Polyclonal Antibody

CAT: 0498-bs-4055R-01Size: 20 µLDry Ice: NoHazardous: No
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CAT#:0498-bs-4055R-01Size:20 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC) . Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.
Synonyms
PPAR; NR1C1; hPPAR; PPARalpha; Peroxisome proliferator-activated receptor alpha; PPAR-alpha; Nuclear receptor subfamily 1 group C member 1; PPARA
Gene ID
5465
Swiss Prot
Q07869
Modification Site
Ser12
Cellular Locus
Nucleus
Host
Rabbit
Cross Reactivity
Human, Mouse, Rat, Rabbit
Target
Phospho-PPAR alpha (Ser12)
Clonality
Polyclonal
Isotype
IgG
Conjugation
Unconjugated
Source
KLH conjugated synthetic phosphopeptide derived from human PPAR alpha around the phosphorylation site of ser12
Applications
WB, ELISA, FCM, IHC-P, IHC-F, IF (IHC-P), IF (IHC-F), IF (ICC)
Purification
Purified by Protein A.
Concentration
1µg/µl
Dilution
WB (1:300-5000), ELISA (1:500-1000), FCM (1:20-100), IHC-P (1:200-400), IHC-F (1:100-500), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)
Buffer
0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Modification
Phosphorylation
Storage Conditions
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Gene ID URL
5465
Predicted Cross Reactivity
Dog, Cow, Pig, Horse, GuineaPig

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