Vimentin Polyclonal Antibody, Cy3 Conjugated

• Background:

  Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

• Synonyms:

  Vimentin; VIM

• Gene ID:

  7431

• Swiss Prot:

  P08670

• Cellular Locus:

  Cytoplasm

• Host:

  Rabbit

• Cross Reactivity:

  Human

• Immunogen:

  101-200/466

• Target:

  Vimentin

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Conjugation:

  Cy3

• Source:

  KLH conjugated synthetic peptide derived from human Vimentin

• Applications:

  WB, FCM, IF (IHC-P), IF (IHC-F), IF (ICC)

• Purification:

  Purified by Protein A.

• Excitation Emission:

  512,550nm/570,615nm

• Concentration:

  1µg/µl

• Dilution:

  WB (1:300-5000), FCM (1:20-100), IF (IHC-P) (1:50-200), IF (IHC-F) (1:50-200), IF (ICC) (1:50-200)

• Buffer:

  Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

• Modification:

  Unmodified

• Storage Conditions:

  Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

• Gene ID URL:

  7431